1/20/2023 0 Comments Air temperature inside autoclave![]() ![]() This pure steam is called saturated steam. Inside the autoclave, all air is removed and only steam remains. Steam can be produced either inside the chamber or externally by a steam generator. Once we close the door and set the autoclave to the appropriate cycle, water begins to enter the chamber and heat up to 121℃ or 134℃, thus creating steam. The load refers to whatever we wish to sterilize, i.e., surgical instruments, as well as laboratory essentials like solids, liquids, or hollows. We open the door, and place the load inside the autoclave chamber. We will look at three main reasons: heat, pressure, and biology.īut first let’s just review what happens in the autoclave. Now let’s take a look at why steam is considered the best sterilization agent, or sterilant, for autoclaves. In our previous post, we explored the basic concepts of vapor, steam, boiling, evaporation, and condensation. ![]() 5th post in series, "Autoclave Sterilization Basics" ![]()
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